AMPA Receptor Structure, Function, and Dynamics

Shisa proteins and AMPA- receptor function

Structure and function of reconstituted AMPA receptors

Background: Ionotropic glutamate receptors mediate fast excitatory synaptic transmission in the vertebrate CNS. Results: Conformational flexibility and dimensions of functional membrane-embedded full-length GluA2flop receptors are characterized by atomic force microscopy. Conclusion: Conformational flexibility and dimensions are both strongly affected by receptor density. Significance: The reco...

Transmembrane AMPA receptor regulatory proteins and AMPA receptor function in the cerebellum

Heterogeneity among AMPA receptor (AMPAR) subtypes is thought to be one of the key postsynaptic factors giving rise to diversity in excitatory synaptic signaling in the CNS. Recently, compelling evidence has emerged that ancillary AMPAR subunits-the so-called transmembrane AMPA receptor regulatory proteins (TARPs)-also play a vital role in influencing the variety of postsynaptic signaling. This...

Structure and Dynamics of AMPA Receptor GluA2 in Resting, Pre-Open, and Desensitized States

Ionotropic glutamate receptors (iGluRs) mediate the majority of fast excitatory signaling in the nervous system. Despite the profound importance of iGluRs to neurotransmission, little is known about the structures and dynamics of intact receptors in distinct functional states. Here, we elucidate the structures of the intact GluA2 AMPA receptor in an apo resting/closed state, in an activated/pre...

Regulation of synaptic structure and function by palmitoylated AMPA receptor binding protein.

AMPA receptor binding protein (ABP) is a multi-PDZ domain scaffold that binds and stabilizes AMPA receptor (AMPAR) GluR2/3 subunits at synapses. A palmitoylated N-terminal splice variant (pABP-L) concentrates in spine heads, whereas a non-palmitoylated form (ABP-L) is intracellular. We show that postsynaptic Sindbis viral expression of pABP-L increased AMPAR mediated mEPSC amplitude and frequen...